Ischemia Care Biomarkers of Acute Stroke Etiology (BASE) "BASE"

Recruiting

Phase N/A Results N/A

Trial Description

The proposed study will validate the clinical use of new biomarker blood tests to identify blood components that may differentiate between diverse stroke etiologies and clinical outcomes as listed below:
1. Differentiate between cardioembolic and large artery atherosclerotic ischemic strokes, when hemorrhagic stroke is ruled out.
2. In cases of ischemic strokes of unknown or "cryptogenic" etiology, determine the ability of biomarker blood tests to predict etiology between cardioembolic and large artery atherosclerotic.
3. In cases of cardioembolic ischemic stroke, further differentiation of cardioembolic ischemic strokes into those caused by atrial fibrillation (AF) and those not caused by AF.
4. Differentiate "transient ischemic attacks" (TIAs) from acute ischemic strokes.
5. Differentiate TIAs from non-ischemic "transient neurological events" (TNE) with similar symptoms.

Detailed Description

Acute ischemic stroke (AIS) is a leading cause of adult mortality and morbidity in the United States, affecting over 800,000 individuals, annually, leaving many with permanent disability. Furthermore, hundreds of thousands of Americans experience a transient ischemic attack (TIA), a momentary episode of neurologic dysfunction, which often precedes a major stroke and serves as a warning for future ischemic events. Despite symptoms resolving, experiencing a TIA increases the risk of stroke by 20% within 90 days. Emergent evaluation, prompt acute treatment, and identification of stroke etiology for secondary prevention are key to decreasing the morbidity and mortality associated with cerebrovascular disease. Key to treatment and prevention is the identification of stroke etiology - large vessel atherosclerosis, cardioembolic phenomenon, or in-situ small vessel cerebrovascular disease - since primary and secondary prevention measures differ based on stroke subtype. The diagnosis of ischemic stroke includes a combination of patient history, clinical assessment, and brain imaging. However, identifying the cause of cerebrovascular ischemia is challenging and routinely assigned of cryptogenic origin.
Therefore, there is a great need to understand the pathogenesis of TIA and AIS events in order to develop more effective preventative measures. Recent studies have identified the differential expression of genes in whole blood that may differentiate the major ischemic stroke types. Such differences may help identify TIA and AIS events that are more likely to respond to therapy specifically tailored to the major stroke type. Furthermore, by establishing a more robust standard for secondary prevention, future stroke events may be avoided.
BASE is a multisite prospective study with a estimated enrollment of up to 1100 subjects adult subjects and 100 age, gender and co-morbidity matched controls ("Controls") will be recruited from patients who present to the Emergency Department (ED) or hospital with suspected AIS or TIA. Research personnel will identify potential patients by responding to "Brain Attacks" pages from the ED to the Stroke Team for patients who meet current Brain Attack criteria. Following evaluation by the ED and neurology physicians, the clinical coordinator will verify the patient had a suspected AIS or TIA and meets eligibility criteria. The patient or their legal surrogate will be approached for study participation. Written informed consent will be obtained for all subjects enrolled.
There are two recruitment windows related to BASE determined by time of symptom onset, time of presentation at ED or hospital, and ability to consent:
1. "BASE" - patients that present with suspected stroke symptoms within 18 hours of symptom onset or last known normal time OR
2. "BASE 24" - patients that present within 24 hours +/- 6 hours (i.e. 18 - 30 hour window) of symptom onset or last known normal time and clinical evidence suggesting Acute Ischemic Stroke.

Conditions

Interventions

  • Biomarker blood draw Other
    Other Names: PAX Gene Blood RNA tube, PreAnalytiX, Germnay
    Intervention Desc: Comparison of gene expression profiles using RNA isolated from whole blood.
    ARM 1: Kind: Experimental
    Label: Ischemic Stroke
    Description: Ischemic stroke subjects will have 2 PAX Gene Blood RNA tubes drawn at 8 hours of onset of symptoms upon arrival to the Emergency Department or hospital; 24 hours +/- 6 hours from symptom onset and 48 hours+/- 6 hours from symptom onset or discharge from the hospital, whichever comes first. Biomarker blood draw
    ARM 2: Kind: Experimental
    Label: TIA (Transient Ischemic Attack)
    Description: TIA subjects will have 2 PAX Gene Blood RNA tubes drawn at 8 hours of onset of symptoms upon arrival to the Emergency Department or hospital; 24 hours +/- 6 hours from symptom onset and 48 hours+/- 6 hours from symptom onset or discharge from the hospital whichever comes first. Biomarker blood draw
    ARM 3: Kind: Experimental
    Label: Non-Ischemic TNE
    Description: Non-Ischemic Transient Neurological Event (TNE) subjects will have 2 PAX Gene Blood RNA tubes drawn at 8 hours of onset of symptoms upon arrival to the Emergency Department or hospital. Biomarker blood draw
    ARM 4: Kind: Experimental
    Label: Control
    Description: Control group subjects will have 2 PAX Gene Blood RNA tubes drawn within 8 hours of arrival to the Emergency Department or hospital. Control group matched with ischemic stroke and TIA subjects for age, race, gender, smoking history with at least one of the following vascular risk factors: diabetes, hypertension, atrial fibrillation, hyperlipidemia. Biomarker blood draw

Trial Design

  • Observation: Cohort
  • Perspective: Prospective
  • Sampling: Probability Sample

Trial Population

Subjects eligible for enrollment include: 1. Ischemic stroke within 24 hours of symptom onset. 2. Transient Ischemic Attack (TIA) within 24 hours of symptom onset. 3. Non-ischemic transient neurologic event (TNE) within 24 hours of onset. 4. Normal controls that will be non-neurologic patients who are matched with the other ischemic stroke and TIA patients for age, race, gender and smoking plus one or more of the following vascular risk factors: diabetes, hypertension, atrial fibrillation, hyperlipidemia.

Outcomes

Type Measure Time Frame Safety Issue
Primary RNA gene expression in peripheral blood. Up to 60 days. No
Primary Cardioembolic and large vessel stroke stiology Up to 60 days.
Primary TIA differentiation from non ischemic events (TNE). Up to 60 days.
Secondary Cryptogenic stroke Up to 60 days.
Secondary Atrial fibrillation and stroke Up to 60 days.
Secondary Stroke and TIA, differentiation Up to 60 days.

Biospecimen Retention:Samples Without DNA - Biospecimen will be whole blood samples.

Sponsors